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Samples were taken at intervals of 12 or 24 hours and each endogenous experiment lasted for 168 hours.
For the anaerobic endogenous experiment, activated sludge mixed liquor taken from the parent reactor was placed in glass flasks directly with an initial nitrogen gas purging for 5 minutes.
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Endogenous experiments were carried out under both anaerobic and aerobic conditions to examine dynamics of polymers of PAOs.
For the aerobic endogenous experiments, activated sludge mixed liquor taken from the parent SBRs was aerated constantly.
Notably, ectopically expressed UXT impaired the interaction between NICD and RBP-Jκ, as demonstrated by the endogenous immunoprecipitation experiment (Fig. 6E).
Endogenous coimmunoprecipitation experiments indicated that RIG-I did not interact with REUL without stimulation, but was associated with REUL after SV infection.
Indeed we demonstrate robust B-Raf and C-Raf heterodimerization in endogenous immunoprecipitation experiments.
All endogenous FRAP experiments were performed using homozygous ds-EGFP or ft-EGFP.
For the endogenous gene experiments, sgRNAs were cloned by oligo cloning method mentioned above into a PBneo-sgRNA expression vector sgRNA target sequences, oligos for cloning are listed in Supplementary information, Table S1.
In a typical experiment, endogenous SIRT1 was immunoprecipitated from 293T cells using the rabbit anti-SIR2 antibody.
We next performed an endogenous BECN1-PARK2 coimmunoprexperimentn experinenthen the presence or absence of CCCP.
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