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Gapdh was used as the endogenous control.
Data were normalized to Gapdh expression, as an endogenous control.
The small RNA RNU6 was used as endogenous control.
The levels of 28S rRNA served as an endogenous control.
We used GAPD (Gapd) as the endogenous control.
Relative gene expression was normalized to the endogenous control GAPDH.
GAPDH (Hs99999905_m1) was used as the endogenous control.
In each plate, endogenous control, samples and negative controls were analyzed in triplicate.
The PCR primers for GAPDH was also designed to serve as the endogenous control.
The mean Ct value was calculated for each gene and normalized to the endogenous control.
Relative quantification was performed in a multiplex reaction using β-actin as endogenous control.
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