This observation correlates with our previous results, showing that kerp1 anti-sense RNA transcription in E. histolytica does not lead to a reduction of the full-length, endogenous mRNA abundance, unless amoebae are stressed [12].
To better define the roles of hnRNP A1, hnRNP H, FUS/TLS and p68 in regard to p19 as well as other genes, we analyzed the effect of depleting these four splicing factors by means of RNAi-mediated depletion assays to determine how (1) endogenous p19 abundance is affected and (2) the expression of other genes is regulated.
After passing R-Luc quality control, we then determined the global endogenous cytosine abundance.
On the other hand, inhibition of the MAPK pathway dramatically increased endogenous FBW7 abundance.
We also observed overall patterns of endogenous retroviral abundance that, under a neutral model, are not universally male biased.
To analyze changes in endogenous protein abundance during osteogenesis, USSC were cultivated on 6-well plates and induced with DAG as described above.
Another protocol that uses a set of "spike-in" calibration standards was proposed to estimate, but not to directly measure, endogenous transcript abundance [ 9].
A relationship between body size and parasite species richness is thus possible, for example it has been shown that endogenous retroviruses abundance negatively correlates with body mass [ 93].
In all stranded RBS-seq libraries, global endogenous cytosine abundance was less than ~1 % compared to ~22%% for non-BS treated RNA-seq samples (Additional file 1: Figure S1B, S1C).
There is clearly a technical challenge in studying endogenous low abundance membrane proteins such as Orai3 and so, while we confirmed the specificity of our anti-Orai3 antibody for studies of endogenous Orai3 in endothelial cells, it was important to test our hypothesis without using this antibody.
