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The episode faced criticism from the crew over its ending using a deus ex machina to indicate that the Kindred may have been aliens.
The fragment was then ligated into a pUC18 vector, which had been prepared by EcoRI and HindIII restriction digestion and by blunt ending using the Klenow fragment.
The 35S fragment was obtained by digesting vector pART-7 first with NotI and, then blunt ending using T4 DNA polymerase followed by digestion after gel purification with XbaI.
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(b) Long read paired ends using the Roche/454 FLX platform.
Primary repair of midsubstance tears are repaired end to end using a 3-0 non-absorbable suture.
HindIII site was blunt ended using Klenow reaction.
Double stranded cDNA templates were blunt ended using End-It™ Repair Kit (Epicentre®).
Adaptors were blunt-end ligated to the fragment ends using T4 DNA ligase.
Clip the excess wire ends using wire cutters.
The warning ends, "Use this helmet at your own risk".
The particular size applied relates to end-use.
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