Sentence examples for ended using from inspiring English sources
The Navy did not identify the couple, but said the man reported the accusation to Navy officials on Tuesday, two days after the convention ended, using a telephone hot line set up to handle sexual harassment complaints after the association's raucous convention in 1991.
All entry experiments were ended at the times indicated by the addition of 4% paraformaldehyde (PFM), with the exception of those involving transferrin which were ended using 4% PFM with 0.25% glutaraldehyde.
To reduce the length of the vector sequence between the T7 promoter in the pGEM-T vector and the inserted DNA fragment, the plasmid pGEMglB was digested with the restriction enzymes ApaI and SacII, purified from an agarose gel using Ultrafree-MC columns, blunt ended using DNA polymerase I Klenow fragment, and then re-ligated using T4 DNA ligase.
HindIII site was blunt ended using Klenow reaction.
Double stranded cDNA templates were blunt ended using End-It™ Repair Kit (Epicentre®).
The EcoRI and AgeI sites were blunt ended using Klenow enzyme.
(b) Long read paired ends using the Roche/454 FLX platform.
Primary repair of midsubstance tears are repaired end to end using a 3-0 non-absorbable suture.
(2) Subcloning of p35 expression cassette into pShuttle-2B6-IHOR: To obtain a blunt-ended p35 expression cassette driven by a hEF1-HTLV promoter, pORF-p35 was digested with BfuA I then blunt-ended using Klenow enzyme.
Samples were then treated with Bst DNA Polymerase (New England BioLabs) to repair the ends, A-tails were added to the sheared ends using Klenow 3′-5′ exoNew Englandew England BioLabs), and the samples blunt-ended using a Quick Blunting Kit (New England BioLabs).
Adaptors were blunt-end ligated to the fragment ends using T4 DNA ligase.
