Sentence examples for end transcript from inspiring English sources
To this end, transcript levels were expressed as ratios of the lesional value to the basal level observed in the corresponding sample of normal mucosa.
Clear signatures of 3′ end transcript polyadenylation were found for 10 of the 12 PCGs encoded on the H-strand (Fig. 4).
Probes of 54-57 nuclengthe length (plus 652 unrelated human probes) have been designed using the 3' end transcript region templates and spotted in four replicates to prepare a new DNA microarray platform, namely a M. galloprovincialis Immunochip.
A cDNA normalization was used to improve coding sequence coverage, avoid AT homopolymer artifacts, and reduce excessive 3' end transcript sequence [ 39]. cDNAs from both libraries were amplified using the Clontech Advantage HF system (Clontech Laboratories , Inc and normalized utilizing the Evrogen Trimmer cDNA Normalization kit (Axxora, LLC).
End-to-end transcript levels were quantified with the aid of a Fuji phosphorimager (and Aida software).
The genomic organization of AGAP006442 and AGAP003387, which produced the most abundant genic siRNAs, does not show any 3′-end transcript overlapping (VectorBase).
Paired-end transcript sequences were mapped against the International Tomato Annotation Group ITAGG) Solanum lycopersicum protein reference version 2.3 (http://solgenomics.net) with SOAPaligner/SOAP2 [ 29].
It should be noted that differential expression of 3'-end transcript isoforms was easily identified using the SAGE method, as also demonstrated with the ccnb2 transcript.
Proprietary high-end transcript profiling systems such as Massive Parallel Signature Sequencing [ 1] and GeneCalling [ 2] are excellent tools for genome-wide quantitative transcription analysis on any organism, but they are available to only a small fraction of researchers.
CAGE technology captures the cap structure in the 5′-end of mRNAs before they are processed and sequenced, resulting in genome-wide 5′-end transcript signatures to obtain precise TSS corresponding to the expression levels [ 30, 31].
The sequences of 5'-end transcripts are important for finding the signals for initiation of transcription.
