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To this end, the coding sequence for DsRed.T4 in pGS.pA+.DsRed [28] was replaced by that of Rep68 giving rise to construct pGS.pA+.Rep68.
To this end, the coding sequences of stp1 and stk1 were replaced with genes that conferred either chloramphenicol (Cm, [64]) or kanamycin resistance (Kn, [65]) in S. aureus RN4220 and subsequently transduced into S. aureus Newman (see Methods for details).
To this end, the coding sequence for EGFP from pEGFP-1 (Clontech, Palo Alto, CA, U.S.A) was digested with BamHI and XbaI and ligated in frame with the LacZ-α-peptide of the broad-host-range vector pBBR1MCS-2 [48] previously digested with the same enzymes, rendering the plasmid pBBR1MCS-2EGFP.
To this end, the coding region of ScFKBP12 was placed under the control of the constitutive CaMV 35S promoter and introduced into Arabidopsis (ecotype Columbia) through Agrobacterium-mediated transformation.
To this end, the coding region of BnaMKK2,-3,and -4 as well as BnaMPK3, -5, -6, -9 were fused to the N-terminus of green fluorescent protein (GFP) reporter gene with a Gly-Ala rich peptide linker between CDSs and GFP in a binary vector.
Similar(55)
"We need more people to come forward and end the code of silence," Mr. Daley said at a recent news conference on the city's crime-fighting efforts.
He cited "Who's Afraid of Virginia Woolf?," the acclaimed adaptation of Edward Albee's Broadway play and director Mike Nichols' directorial debut, as the "film that ended the code.
The ; (semicolon) ends the code.
A multi-criterion stopping rule was developed and successfully implemented to end the inverse code at the solution.
Makary, 42, aims to end the professional code of silence that allows colleagues like Dr. Hodad to thrive.
He ended the dress code that required men to wear ties and jackets.
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