Exact(3)
We placed one probe on the center of the plate (where worms begin the assay) and one probe on the edge of the plate (where worms normally end the assay).
To this end, the assay previously used to analyze cultured cell extracts [ 9, 21] was adapted to evaluate photosynthetic tissue samples.
To this end, the assay was performed using genomic DNA derived from human cancer cell lines before and after 5-Aza treatment and with M-WGA and unmethylated WGA DNA (U-WGA) as controls (Fig. 3).
Similar(57)
At the end of the assay, a melting curve was constructed to evaluate the specificity of the reaction.
At the end of the assay 7AAD (which binds to DNA and can only enter membrane-compromised cells) is added to measure cell death.
Strikingly, no killing was observed in the wild type, but rather a slight increase in colony forming units (CFU) towards the end of the assay.
In experiments using the MAPK pathway inhibitor, SB203580, the reagent was added 4 h after transfection and remained until the end of the assay.
To exclude effects of the incubation conditions on the viability of the bacteria, serum-free controls were run in parallel and CFUs determined at the start and end of the assay (data not shown).
We have described in a separate paper a model sandwich assay in which ≈1×105 to 1×106 PMPs (2.8 μm diameter) are immobilised on a plastic strip at the end of the assay.
The design of experiment with response surface methodology and multiple optimization response was used to maximize the recovery of fluorescent probe at the end of the assay simultaneously with the optimization of target probe binding.
In the group of eight mice that received 22.2 MBq 131I-labeled B-B4 mAb, five achieved a CR and three a PR (duration of 48 ± 32 days), and three mice were cured at the end of the assay.
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