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This can be attributed to two factors: First, we are computing local alignments, so we can always choose to end the alignment subtrees in the nodes u, v. Second, there are many different labels found at the edges (or nodes) of a fragmentation tree.
Holding the string tight at the other end, the alignment of the semi-circle is defined by swinging the end of the string from one tip to the other.
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This problem is exacerbated by the need for flanking nonrepetitive "anchor" sequence to accurately start or end the repeat alignments in the correct position, as this further reduces the remaining read length that can span into the repeats.
Since a large portion of trnH-psbA sequences was missing data at one or both ends, the alignment was trimmed and 11 taxa with missing trnH-psbA data were removed from the BLAST analysis; the rbcL alignment did not require trimming, but three taxa with missing rbcL data were also removed (Supporting Information Table S2).
Because the sequences contained many repeats near their 3′ ends, the alignment was uncertain in the respective region and contained many gaps.
Sometimes, regions at the beginning or end of the alignment will be poorly aligned because the sequences were too dissimilar in length.
It is important to know if the sequencing experiment was single-end or paired-end, as the alignment software will require the user to specify both FASTQ files for a paired-end experiment.
At the end of the alignment (Panels A and B) a black line marks the end of the protein including the peptide.
Both Colubraria contigs appear to be incomplete, with comp1091011_c0_seq4 having a predicted signal peptide but missing the C-terminal tail and comp109011_c1_seq2 aligning with the C-terminal end of the alignment.
Indels in homopolymers at the end of the alignment led to failure to find some correct alignments (the observed start and end positions are not the expected ones).
BLAST performs local alignment, returning the start and end of the alignment (the hit) for the query sequence and the target sequence, as well as the E-value.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com