Sentence examples for end of transcript from inspiring English sources

Second, random primers used in the second cycle have dubitable uncertainties that, unlike the oligo (dT) primer, can bind not only to 3' poly(A) end of RNA, but also to the middle or the other end of transcript, concluding cDNA's uncompleted reverse transcription from amplified RNA, which leads greater bias after the second cycle amplification.

Nearly half of all TE annotations (46.6%) encompass only the 5′ or 3′ end of transcript contigs (Additional file 1: Figure S7), suggesting that transcription initiation or termination can occur within TEs.

What they call Bushwick now? [East Williamsburg. And it starts around Rochester now, it's crossed Brooklyn.] End of transcript.

S: Blow it out your... (End of transcript).

When comparing results of 0.1 µg and 10 µg RNA, skew in target counts is evident on the low end of transcript abundance, but there is linear concordance for moderate and high abundance transcripts (Figure 2F).

But when amplicons were very close to 3' end of transcript, the DP would increase again because of 3' degradation.

Additionally, usage of poly dT priming introduces a bias towards overrepresentation of the 3' end of transcripts, particularly in the case of large transcripts.

In addition, the use of oligo dT primers, instead of random hexamers, can be biased towards the 3′ end of transcripts.

Biased amplification of the 3′ end of transcripts does not have a major impact on the overall transcript profile due to the 3′ bias of probe sets incorporated in the array design.

Primers specific for the 3′ end of transcripts were designed by use of PRIMER EXPRESS 3.0 (Applied Biosystems).

In most cases the signal intensities at the 3' end of transcripts were greater when poly(A)+ RNA was analyzed as compared to 5' capped RNA.