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We modified the TEL construct used in Figure 5H by adding a 21-bp DNA duplex next to the 3′ end of the TEL G4 sequence (referred to as TEL-DD21).
Finally, in order to compensate for the expected TEL benefit, appropriate procedures of compensation were designed for the students in the Re-study group (e.g. the integration of curricular activities at the end of the TEL program).
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Addition of a DNA duplex next to the 3′ end of TEL does not affect the periodic G4 unwinding, indicating that Pif1 monomers unwind G4 but do not go beyond to unwind dsDNA.
Addition of a 21-bp DNA duplex next to the 3′ end of TEL did not affect the periodic G4 unwinding, indicating that even though a Pif1 monomer repetitively unwinds G4 DNA with a very high yield, it does not go beyond it to unwind dsDNA.
Thus, this approach separately takes into account biological considerations related to the severity of the end point (via the TEL), statistical considerations related to the study data (via the UERSNCD), and adjustments from the test species to sensitive humans (via uncertainty factors or chemical-specific adjustments).
Similar to the observations for TP, deletion of the ssDNA region at the 5′ end of TEL inhibited Pif1 loading and G4 unwinding in K+.
TEL AVIV — At the end of the third U.S. presidential debate — the one in which Barack Obama and Mitt Romney clashed at times over actual foreign policy issues — I had to reconcile two competing feelings.
Despite the widespread application of the TEL, three related drawbacks must be mentioned.
Establishing micro-macro link Fig. 2 Overview of the TEL environment and local instructional theory.
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