Exact(4)
This is used to catalyse the attachment of A overhangs to the 3' end of the sequencing fragments so as to complement 5' T overhangs on the platform specific adapters.
A sequence was considered as a potential new isoform if the region left unmasked was at one end of the sequencing read and was greater than 10 bp long, or it was inside the masked sequence.
We clipped out the 3' adaptors form the reads obtained at the end of the sequencing run using the FASX-toolkit from Hannon lab (http://hannonlab.cshl.edu/fastx_toolkit/) before further analyses.
As the MCPyV VP1 PCR product was too short for direct sequencing, we added a 40 base pairs (bp) nonspecific nucleotide tail [ 34] (Table 1) in addition to a poly(C) to the 5′ end of the sequencing primers to accomplish a product of 110 bp.
Similar(56)
By the end of the sequence, the double bassist is literally running rings round her instrument.
Her perfectly judged glissando sweeps at the end of the sequence will stay in the memory.
"In reality, the Palestinians were ignored," Curtis narrates toward the end of the sequence.
In England, at the end of the sequence of the 30s, you had the grand leadership, which was Churchill.
So, at the end of the sequence, I will probably have tens of thousands of words or even hundreds of thousands of words of unused material".
At the end of the sequence of questions, children are asked to create their own masterpiece using the online art tools.
By the end of the sequence, he has top billing with his owner George – even in the world of the movie, he's a star.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com