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Sixty-five of the 71 confirmed Class 1 events showed evidence of a simple break point sequence when aligned to the reference sequence; the five prime end of the sequenced fragment aligns to one region of the genome and the three prime end aligns to region upstream of the location where the five prime end aligns (panel A in fig. 4).
Short read aligners such as BWA align each read independently to the reference genome and are unable to properly align reads that contain an insertion/deletion event close to the start or end of the sequenced reads.
Clone O0059H07, which overlapped at the 5′ end of the sequenced clone, had the expected unique 5′ end and terminated at the EcoRI site at base 93176.
From 58 kb upstream to 5 kb downstream (the end of the sequenced maize BAC clones), the teosinte and maize haplotypes are highly conserved, except for an LTR retrotransposon inserted in maize haplotype about 13 kb upstream of the tb1 coding region.
This includes statistics such as the length, average quality, and GC content distribution of the sequenced reads, as well as the distribution of the sequenced bases and their quality for every read position and detection of overrepresented K-mers at the start and the end of the sequenced reads.
Similar(55)
By the end of the sequence, the double bassist is literally running rings round her instrument.
Her perfectly judged glissando sweeps at the end of the sequence will stay in the memory.
"In reality, the Palestinians were ignored," Curtis narrates toward the end of the sequence.
At the end of the sequence she slithers down the cloth, collecting it under her body.
In England, at the end of the sequence of the 30s, you had the grand leadership, which was Churchill.
There is also no count of credentials or marker at the end of the sequence of credentials; the sequence ends when the file ends.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com