At the end of the labeling, 0.8 mg of EDTA-2Na was added to the mixture solution.
The radiochemical stability of the final radiolabelled product: The radiochemical purity was checked at room temperature 24 h after the end of the labeling and was 99.60 ± 0.35%.
Though Margiela remained elusive, he sold his business to OTB in 2002, the beginning of the end of the label's insider appeal.
As Azada and Falk demonstrate on his exclusive mix for THUMP, there's not much arty, abstract experimentation to distract from the banging, no-bullshit end of the label's catalogue.
The 6-carboxyfluorescein label (6-FAM) was incorporated at the 5' end of the labeled oligonucleotides, and all oligonucleotides were purified by HPLC.
A delay of 1 s between the end of the labeling pulse and image acquisition was inserted to reduce transit-related effects.
A leader sequence (GTTTCTT) was appended to the 5' end of each reverse primer to catalyze the non-templated enzymatic addition of a nucleotide to the 3' end of the labeled product strand [37].
At the end of the labeling period a considerable fraction of the β cells had re-replicated as indicated by CldU+IdU+ double labeling.
At the end of the labeling period, detection of labeled cells was performed using a specific anti-BrdU antibody (GE Healthcare) and the HistoMouse™ MAX kit (Zymed Laboratories), according to the manufacturer's suggestions.
