Sentence examples for end of the inserted from inspiring English sources
In order to determine the minimal sequence requirements for termination-reinitiation in VP2 expresssion, deletions of increasing size were made from the 5' end of the inserted viral information (Figure 1b).
After ethanol precipitation, PCR amplifications were performed according to the protocol instructions both with Pwht1 and Plac1 primers for the 5' end and with Pry4 and Pry1 primers for the 3' end of the inserted transgene.
Variations included partial inversion of the 5' end of the inserted sequence about 20%% (36/195) of inserts where both ends were found by discordant read analysis.
Nucleotide sequences were generated from one end of the inserted DNA in randomly selected clones picked from the UF and MF libraries, resulting in 1,152 and 864 sequence attempts, respectively (Table 1).
The plant genomic sequence adjacent to the 3' nos end of the inserted DNA was used as a starting point of a PCR-walking procedure aimed at obtaining the wt sequence at the RR locus in non-GM material [ 21].
41 RNA-based splicing detection revealed an intronic insertion of 75 nucleotides between exon 1 and 2. Germline sequencing revealed a T>G transversion at intronic position c.212-478, creating a canonical splice donor site at the 3′ end of the inserted sequence.
The plasmid was then linearised by complete digestion at the 5′-end of the inserted PCR-product with the NotI restriction enzyme (Fermentas, St Leon-Rot, Leon-Rot, Germany
The ends of the inserted PCR product were sequenced using the primers provided with the cloning kit for additional verification of FIV Ple cloned products.
