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Sometimes, regions at the beginning or end of the alignment will be poorly aligned because the sequences were too dissimilar in length.
Both Colubraria contigs appear to be incomplete, with comp1091011_c0_seq4 having a predicted signal peptide but missing the C-terminal tail and comp109011_c1_seq2 aligning with the C-terminal end of the alignment.
At the end of the alignment (Panels A and B) a black line marks the end of the protein including the peptide.
The top row shows the results obtained when LRTs of the molecular clock were conducted in 14 windows by starting at positions 1 500, then moving in 500 bp steps (e.g., positions 1 500, 501 1000, etc)., until the end of the alignment was reached.
The last letter t in s2 matches a space at the right end of the alignment.
Gapped regions at the beginning and at the end of the alignment were trimmed.
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For strain accession numbers, see table 1. Unaligned contigs are retained and positioned at the end of the alignments.
The beginning and end of the alignments were evaluated independently, and the difference between the alignment boundaries and the embedded domain boundaries was calculated in number of residues.
Both genomes had low among-sample diversity at the 3' end of the alignments, and an example of strong conservation in an approximately forty-base window was shown in Additional file 1: Figure S1.
We should crop off these poorly aligned regions on the ends of the alignment before moving on.
Phylogenetic analyses were performed using near full-length aligned SSU rDNA sequences, only the extreme 5'-and 3'-ends of the alignment were excluded leaving 1472 sites, using PHYLIP 3.6b [45].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com