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The other end of the adapter is comprised of single stranded, divergent sequences that serve as binding sites for a pair of primers that, after PCR, generate DNA fragments that have different adapter sequences on each end.
The flask was then placed below the condenser and the end of the adapter tube was dipped in the acid.
The same basic design was used for NgoMIV index adapters except for addition of an NgoMIV-specific four base 5′-overhang placed at the end of the adapter.
A corresponding single 'T' nucleotide on the 3′ end of the adapter provides a complementary overhang for ligating the adapter to the fragment.
A single T nucleotide was added on the 3' end of the adapter for ligating the adapter to the cDNA fragments.
Plug the other end of the adapter to an electrical outlet.
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The 10 dB, 50 Ω attenuator is used on each end of the adapters.
The SOLiD protocol allows simultaneous 5' and 3' adapter ligation to the ends of small RNAs, a method based on hybridization of N6 at the end of the adapters.
Plug the USB end of the power adapter cable into the AC power adapter.
Simply plug the PC Adapter card into a notebook, connect one end of the included adapter cord to the PC card, the other end to the BlackArmor PS110 drive, and it is set.
On the basis of qPCR quantification with probes specific to the ends of the adapters (KAPA Biosystems), libraries were normalized to 2 nM, and then denatured using 0.1 N NaOH on the Perkin-Elmer MiniJanus.
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