Sentence examples for end of template from inspiring English sources
We then used terminal deoxynucleotidyl transferase (TdT) to tail the 3′ end of template DNA with oligo deoxyadenosine (dA).
The detail PCR procedures were described in Lo et al. [11] Resequencings for SNP genotyping were performed from either end of template employing the sequencing primers in Table S8.
At the end of template synthesis, NV3Dpol sometimes adds several C using its TNT activity.
In this model, reverse transcription proceeds from the 3' end to the 5' end of template mRNA and often terminates prematurely.
We argue that this is not an annotation artefact, but most likely results from the end of template and reduced random priming potential in the first strand synthesis step amplification.
The nucleosomal population is subsequently submitted to single-end (identifies one end of template) or paired-end (identifies both ends of template) NGS with a varying level of coverage depending on the exact goal of the experiment [ 31].
Telomere attrition occurs with each cell division as a result of DNA polymerase's inability to replicate the extreme 3'-end of template strands [2], [3].
Additionally, we have shown that selective trimming of nucleotide misincorporations that result from post-mortem damage at overhanging ends of template strands could only slightly improve our abi-lity to identify endogenous sequences for Helicos tSMS sequencing data on samples showing high levels of cytosine deamination.
Greenspan was a technocrat's technocrat, a walking, talking cauldron of statistics and factoids, and even though his ideological roots were in the libertarian right, his happy collaboration with Democratic experts in the Clinton administration fitted the end-of-history template perfectly.
Synthesis of plus-strand DNA proceeds to the 5' end of the template and then switches template to the 3' end (circularization) [14].
Our new library preparation protocol utilizes two separate multiplex PCRs (instead of the previous single PCR) during which the sequence adaptors swap positions at the ends of templates.
