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The library was screened with primers designed at the end of sequences of nine points of scaffold gaps where BAC clones cannot be selected to extend the physical contigs.
To address this issue, we identified all sequences from our pyrosequencing run that could be converted into other sequences in the run by deleting one nucleotide anywhere within the sequences (deleted sequences that were sub-sequences of the original were not considered, i.e. deletions within homopolymers in the beginning or end of sequences).
Moreover, an excess of U + G nucleotides occurred from position 17 to the 3′ end of sequences.
Deletion errors tend to occur more frequently in homopolymers and their rates are higher towards the 3' end of sequences.
Sequence C is complementary to a region at the 3′ end of sequences X, X*, Y, and Y*.
Base-calling errors are pseudo-random depending on the platform used and usually more likely to occur towards the 3′ end of sequences.
Similar(51)
The segment sequence is created by selecting next segment to the end of sequence randomly among timbrally most similar segments.
The concentration of inconsistencies at the 3' end of sequence reads has been suggested to be due to accumulation of failures in incorporation of fluorescent dNTPs [8].
As shown in Figure 1, the inconsistent bases found in GA data sets were concentrated around the 3' end of sequence reads.
This motif also marks the end of sequence conservation among members within TIGR03793.
The totally turnaround time from library preparation to the end of sequencing is about 2 days.
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