Sentence examples for end of reverse from inspiring English sources

If you just cheered uncontrollably because you read into my last statement that I am advocating for the end of reverse racism or felt the need to read no further because you felt that I'm merely regurgitating the recent uninspired argument raised by actress Stacey Dash, you may be contributing to America's social stagnation.

Nineteen nucleotides (5'-CACGACGTTG TAAAACGAC-3') were added to the 5' end of reverse primers to allow the incorporation of the M13 fluorescent-labeled primer into PCR products [see 38].

All subjects also noted mild skin reddening where the electrodes were applied, but this also completely disappeared within a few minutes of the end of reverse iontophoresis.

These methods relied on the terminal transferase activity of reverse transcriptase to allow addition of primer sites to the 3' end of reverse-transcribed, first-strand cDNA.

This means that dilution by a factor of 10 to 20 has to be established, assuming that very high organic modifier concentrations are often used at the end of reversed-phase gradients.

Parasites were isolated from these two populations using a 0.2% saponin/1 × PBS treatment and total RNA was purified, treated by RQ1 RNase-free DNase (Promega) and converted to high quality cDNAs using the SMART (switch mechanism at the 5'-end of reverse transcript) PCR cDNA synthesis methodology, as recommended (Clontech).

The T7 promotor was added to the 5′- end of the reverse primer in a second round of amplification (T7- apln-reverse 5′-GTAATACGACTCACTATAGGGAGTTCACTATCTGATGTCAAACCA-3′, (T7- aplnra-reverse 5′-GTAATACGACTCACTATAGGGAGTTCACTATCTGATGTCAAACCA-3′, (T7- aplnrb-reverse 5′-GTAATACGACTCACTATAGGGAGTTCACTATCTGATGTCAAACCA-3′).

The reverse primer was a short 18 22 nucleotide oligonucleotide corresponding to the 5′ end of the reverse HA used to amplify the RT cassette.

Biotin was labeled on the 5′ end of the reverse oligonucleotide.

At the same time, NcoI and BamHI restriction enzyme sites were added to the 3′ end of the reverse primer RNAi-R.

A single point mutation, G-to-A transition, 16 nucleotides from the 3′ end of the reverse primer, was identified to cause allele dropout when using the AmpFℓSTR® Profiler Plus™ primer set.