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While even early studies of chimpanzee probing behavior reported chimpanzees' proclivity to fray the end of probe tools [e.g., Teleki, 1974], it is unresolved whether such tool modifications are intentional or not [Byrne et al., 2013; Takemoto et al., 2005].
Within each well, peaks of parent ions which were within the m/z 800∼3,000 range with intensity above 10 counts ± include/exclude list will be selected automatically for collision-induced dissociation (CID) MS/MS using argon as collision gas and a mass dependent ±5V rolling collision energy until end of probe pattern was reached, starting from the most intense peak.
The basic sensor design was the same as has been previously demonstrated, except that a Fc label was covalently added to the 3′ end of Probe ON-P2.
The syndesmosis being easily accessible for the 3 mm transverse end of probe which could be rotated around its longitudinal axis in all cases during arthroscopy of the ankle joint, confirmed the diagnosis.
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Strong position-dependent effects have been found; mismatches near the middle of probes reduce the signal more than those near the end of probes [ 32, 33].
Known mismatches near the middle of probes are associated with higher average log2 signal ratios (and therefore greater reduction in test strain signal intensity) then those near the end of probes.
We observed that the distance to the 5' end of probes from under-represented genes is significantly smaller than the distance of probes from normally-represented genes (see Figure 5B).
At most, there were 7 nucleotide mismatches originating at either the 3' or 5' end of a probe; probe sequences are provided in Table 1.
Triphenylphosphine ligands were individually coupled through amide bonds to the 5′-end of probe L and 3′-end of probe R (see Scheme S4 and p. 7 in the Supporting Information for details on the purification and characterization).
This is a consistent effect and suggests that the locations of the G-spots on the probes (l = 1 corresponds to the free end of the probe; l = 22 corresponds to the tethered end of the probe) have a direct bearing on the extent of the correlation coefficients between genetically unrelated probes.
Ten bases of thymine were added to the 3' ends of probes that exhibited weak hybridization signals after preliminary testing [ 27].
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