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Synapses typically form between the end of one cell's axon and a dendrite on another cell.
Glutamate released at the end of one cell binds to receptors on the adjacent cell, starting up a signal inside that cell.
To use these open runs, the pseudopod data of all cells from a specific developmental stage were placed in a circular array so that the open end of one cell is closed by the open start of the next cell.
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The biphasic signal became monophasic after the depolarization of one cell end by raised extracellular [K+].
Briefly, following trypsinisation at the end of passage one, cells were counted and suspended in 90% type I collagen gel (2 mg/ml) (Invitrogen), 10%100 × DMEM (Sigma) using 1 × 106 cells/mL.
As shown in Fig. 1 and supplementary material Fig. S2, at the end of one week pulse, cells throughout the polyp were labelled with EdU, as would be expected from hydra cell kinetics.
The cumulative population doublings (cPDL) were calculated using the following equation: cPDL = (log(A) – log(B))/0.301 (A: number of cells at the end of one passage; B: number of cells that were seeded at the beginning of one passage).
Cells were counted by a CASY cell counter (Schärfe Systems, Reutlingen, Germany) when passaged, and cumulative population doublings (cPDL) were calculated using the following equation: cPDL = ]Blog(A) – log(B)]/0.301 (A: number of cells at the end of one passage; B: number of cells that were seeded at the beginning of one passage).
Beginning 10 14 days after transfection, the cumulative population doublings (cPDL) were calculated using the following equation: cPDL = (log(A) − log(B))/log2 (A: number of cells at the end of one passage; B: number of cells that were seeded at the beginning of one passage).
Population doublings (PDL) were estimated using the following equation: n= (log10 F − log10 I)/0.301 (where n is the population doublings, F, number of cells at the end of one passage, and I, number of cells that were seeded at the beginning of one passage).
Cells were counted when passaged and PDLs (population doublings) were estimated using the following equation: n=(log F−log I /0.301 (n represents the number of population doublings, F the number of cells at the end of one passage and I the number of cells that were seeded at the beginning of one passage).
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