At the end of interaction, the reaction was stopped by adding 1 mL of 70% methanol dissolved in PBS for 10 min and washing once with PBS to remove excess of methanol; immediately after, 0.5% safranin dissolved in water was added and incubated for 1 min.
To improve the predictive power of the individual quantitative datasets, we further developed our computational matrix approximation strategy [ 34], and showed that it could transform the original fitness matrices so that these allow for better discrimination of not only negative but also the positive end of interaction spectrum from the background variability [ 31].
In this work, we develop a novel and efficient rank-one matrix approximation method, named QMA, to address the problem of detecting accurately also the positive end of interactions, yet being simple enough for the large-data interaction datasets.
Interestingly, the calling rate also showed a sharp decrease at the end of interactions.
In most experiments, each recording session consisted of a 5 min baseline during which the subject rat was alone in the setup, a 5 min interaction time when stimulus animals/objects were presented across the gap, and another 5 min baseline at the end of interactions.
If both i R and j S are end points of interaction arcs, then one of the arcs subsumes the other one or they are equivalent.
Females mated to cross-taxon males with a smaller aedeagus than conspecific males were able to establish the mating interaction but had issues disengaging at the end of the interaction.
During the experiment, an experienced observer (ET) recorded the number of interactions and the winner of each interaction; the winner was deemed as the octopus that did not retreat by the end of the interaction.
Conventional dual-end depth-of-interaction (DOI) PET detector readout requires two 2D SiPM arrays; with top and bottom SiPM reading the same pixel, there is information redundancy.
All the segments chewed over their ideas yet tended to end inconclusively, sometimes in midthought, as if they had reached the end of productive interaction with that particular line of inquiry.
And even if you end up on the short end of an interaction, I think you're better off in the long run for finding that voice and putting it forward.
