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The promising results on the predictivity of MRD evaluation at the end of induction treatment has raised the need of a new definition of remission.
Ruminal samples were collected at the end of induction (d 10) and during recovery (d 13, 16, and 28), separated to solid and liquid fractions, extracted for DNA, PCR- amplified for the V1-V2 regiof of the 16S rRNA gene, and analyzed for bacterial diversity.
At the end of induction the event free survival was 70% 54-866).
Expression level of the target protein eGFP could be reflected by red fluorescence intensity directly during induction (Fig. 4a) or at the end of induction (Fig. 4b).
A follow-up study of the patient's bone marrow at the end of induction therapy showed no morphologic evidence of residual leukemia and both FISH and chromosome analyses were normal.
At the end of induction, 14/22 (64%) of patients had an objective antitumor response: three patients had a complete response, ten patients had a partial response (greater than or equal to 50% resolution of clinical disease), and one patient had a minor response.
High-risk patients had detectable disease (≥0·01%) at the end of induction.
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The trend remained significant after adjusting for the end-of-induction MRD level (PFG=0.026, HR=1.69, 95% CI=1.07 2.68).
When appropriate, Day 33 (end-of-induction) MRD was further added in the regression model to test the robustness of significant results discovered.
Taking the end-of-induction MRD level into account did not alter the significance (PFG=0.027, HR=2.01, 95% CI=1.08 3.73).
A SNP rs4888024 was found to be associated with end-of-induction minimal residual disease in childhood acute lymphoblastic leukemia from 2 independent cohorts and higher methotrexate clearance [ 47].
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