In these experiments, ferrous and ferric iron concentrations were measured at the beginning and end of experiments with RNA.
Aerobic granules were successfully developed in R2 and R3 while bioflocs dominated R1 until the end of experiments.
We found no difference in survival in both ARDS studies: almost 80% of animals in all groups survived until the end of experiments.
At the end of experiments, mixtures were separated using Amicon ultracentrifugal filters (100 kDa molecular weight cut off; Millipore) and filtrates acidified with 3 mL 1 % nitric acid.
Dissolved oxygen, pH and trace metals (Fe, Al, Zn, Pb, Cd and Cu) were measured at start and end of experiments.
Blood gas parameters, mean arterial blood pressure, and glucose and lactate levels were obtained at the beginning and the end of experiments.
Animals were perfused with formaldehyde and cannulae placement were histologically verified at end of experiments.
At the end of experiments, this aggregate consisted of 25 30 woodlice on average (Figure 8).
Age was verified at the end of experiments using staging criteria by Hamburger and Hamilton [41].
In the end of experiments, the mice were sacrificed by excessive anesthesia.
At the end of experiments animals were killed by an anesthetic overdose.
