After the end of each pointing gesture, the participant was told the name of the target to which he had pointed, either from the addressee in the CP condition or from the computer in the NCP condition.
If the two wing-like springs are pointing to the inside of the trap, turn the springs to the outside of the trap so that the rounded end of each points away from the central "square" jaws.
Like soccer or lacrosse, the game features fluid non-stop play, only pausing the action at the end of each period, after each point, or after a penalty.
Four hours before the end of each time point, 20 μL of 12 mM MTT was added to each well.
At the end of each time point 100 μl of suspension was collected from each petri dish and colony forming units/ml (CFU/ml) was determined by spread plate method on Nutrient agar.
He shredded a piece of a plastic into tiny strands, knotting the fabric together at the end of each metal point.
At the end of each time point, cells were washed twice with PBS and with DMEM supplemented with 3% stripped FCS with or without 17βE2 for variable periods of time.
At the end of each time point, culture supernatants were discarded.
At the end of each time point, pERK1/2 protein levels were evaluated using western blot analysis.
