Sentence examples for end of each extension from inspiring English sources

Fluorescence was measured at the end of each extension.

At the end of each extension phase fluorescence was observed and used for quantitative measurements within the linear range of amplification yielding calculated concentrations as relative units.

Sequencing the 3' end of each extension product should produce the same sequence, regardless of the length of the extension product.

The PCR reaction was stopped after a total of 40 45 cycles and at the end of each extension phase, fluorescence was observed and used for quantification within the linear range of amplification.

The amplification program included the initial denaturation step at 95°C for 10 min, 40 cycles of denaturation at 95°C for 10 s, and annealing and extension at 60°C for 1 min. Fluorescence was measured at the end of each extension step.

All qPCR runs were done using the following conditions: 10 min at 95°C followed by 35 cycles of denaturation (5 sec at 95°C), annealing (5 sec at 62°C), and extension (20 sec at 72°C) with a single acquisition of fluorescence levels at the end of each extension step.

At the end of each knee extension, the LB movement was measured as the distance from 0 cm to the laser pointer mark.

The signal emitted was detected at the end of each annealing-extension step.

Samples were incubated at 95°C for 15 min, followed by 40 cycles of denaturation, annealing and extension at 95, 60 and 72°C, respectively, with fluorescence recorded at the end of each annealing and extension step.

Fluorescence signal was measured at the end of each annealing and extension step at 60°C.

Fluorescence produced by SYBR® Green was measured at the end of the extension step of each cycle.