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Samples for bacterial abundance were collected at the beginning and at the end of each experiment.
At the end of each experiment, the rat was anesthetized and received an ICV injection of Evans blue solution (5%).
We injected 5% Chicago Sky Blue dye (100 nl) at the end of each experiment to identify probe placement.
AlCl3end of each experiment the 2024Al-T4 surface was covered with a black film.
Input resistance (Ri) was monitored at the beginning and the end of each experiment by applying a 10 or 15 mV hyperpolarizing step.
At the beginning and end of each experiment, the skin temperature of each participant was measured by using thermography (Fig. 8).
At the end of each experiment, cultured cells were either fixed in paraformaldehyde or lysed and RNA-extracted or used for culture medium lactate measurements as needed.
Withdrawn blood was reinfused at the end of each experiment.
At the end of each experiment the mixture was filtered through a Whatman filter paper.
At the end of each experiment, animals were euthanized by rapid injection of Euthasol®.
The response variable is obtained at the end of each experiment.
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