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Trastuzumab binds amino acids 579 to 625 at the C-terminal end of domain IV of the extracellular region of HER2 [29].
Pro242 is located near the end of domain 2 of Munc18-1 strucloselly close to Glu379 and thus one could speculate that this exposed region of the protein is involved in Mint binding.
The end of domain D is indicated by the letter D in a circle.
In contrast, the first approximately 50 base pairs at the 5' end of domain I were extremely divergent within individuals.
Multiple mutations in the C-terminal end of domain IV of the translation elongation factor EF-G provided low-cost resistance to kanamycin.
Domain I is spatially located between domains II and III with the fusion peptide lying at the distal end of domain II.
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All the three mutations are present at the carboxy-end of Domain 4 of EF-G, the tip of which interacts with the decoding site of the ribosome (Supplementary Fig. S9).
Unfortunately, in practice, other than the obvious removal of signal peptides, simple transmembrane helices and non-globular segments that flank either the beginning or the ending of domain models [ 3, 27– 31], it is unrealistic to create domain models totally without the remnant residues since structural segments are naturally stitched by inter-linkers.
This study offers preliminary patient-based evidence; patients' use their own vocabulary to directly and tacitly communicate their end-of-life domain needs through narratives.
In multi-domain proteins, the domains almost always occur end-to-end, i.e., one domain follows the C-terminal end of another domain.
DOI: http://dx.doi.org/10.7554/eLife.00435.005 Once the individual domains were fitted, the bond geometry between the carboxy end of one domain and the amino end of the next domain were regularized.
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