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The gathering of authorities around a given question is for Peter one of the main purposes of his work, to some degree an end in itself, while for Aquinas those citations serve the end of constructing an answer to the question.
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This further reinforced the need to consider hooks as an alternative to pedicle screws in bone where risk of fracture is particularly high, especially at the end of constructs, where cantilever forces are greatest.
C28/I2 chondrocytes were transfected with the wild-type COL9A1 construct in the CpG-free vector (C9-WT) or with deletions from the 5′ end of constructs C2 (−565 to +130 bp) and C3 (−169 to +130 bp) and pRL-SV40 vector as an internal control and were then harvested for the luciferase assay.
The information functions across the construct showed that the items were informative across the construct except at the highest end of the construct i.e. those with very high impairment.
The total and individual item information functions showed good information across the construct except at the lowest end of the construct i.e. those with very low activity limitation.
A Kozak sequence and V5-tag were added to the 5' end of the construct by PCR and subcloned into the pBluescript construct using the XhoI site and an internal HindIII site.
Another important avenue for future research is the revisiting of presence of meaning in life as a construct, in particular with regards to the higher end of the construct continuum.
We suspect that if our construct had a longer coiled-coil on the C-terminal end of the construct, the turn in the HIN2 domain would have been ordered.
The 3' end of the construct, containing a 6.4 Kb fragment that spanned the 3' end of intron 6 to the 5' end of exon 9, was amplified with primer pair HPRT LongArm, forward 5'-CTAGCTAGCGGATTTTGAGCCCCCTTACA-3' and HPRT LongArm, reverse 5'-CTAGCTAGCTGGCCACAGAACTAGAACATTGA-3'.
The 5' end of the construct, containing a 3.9 Kb fragment that spanned the 3' end of intron 5 to the 5' end of exon 6, was amplified with the primer pair HPRT ShortArm, forward 5'-GGAAGATCTTAATGGTGGACTTGTGTTCTAA-3' and HPRT ShortArm, reverse 5'-GGAAGATCTCCTGACCAAGGAAAGCAAAG-3'.
This fracture was at the distal end of the construct.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com