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Many talk about moving China beyond the dead end of assembling devices for other companies.
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TR-22A and TR-27A were also found at the end of assembled chromosomes (Table 3), so the possibility to map them by FISH is quite high.
In addition, we created artificially fragmented protein sequences from E. coli to confirm the accuracy of KO assignment to the truncated proteins because the gene products predicted from the end of assembled contigs and singletons were often truncated in the sequences produced by 2nd generation DNA sequencer.
Finally, oligonucleotides designed from the sequence of the ends of assembled contigs were used to obtain single closed contigs [ 61].
For the assembled contigs of the genome of AltDE1, oligonucleotides designed from the sequence of the ends of assembled contigs and PCR amplicons were generated and sequenced to obtain one single closed contig.
Combination of Geneious Pro 5.0.1 (with default parameters) using previously Alteromonas assembled genomes as a reference [ 9, 11, 18] and oligonucleotides designed from the sequence of the ends of assembled contigs were used in order to obtain one single closed contig.
Single nucleotide polymorphisms with a MAF higher than 0.4 very likely break the contig extension; for this reason, they will only be detected on the tailing ends of assembled contigs.
Combination of Geneious Pro 5.0.1 (with default parameters) using previously assembled genomes AltDE and AltDE1 as a reference (Gonzaga et al. 2012) and oligonucleotides designed from the sequence of the ends of assembled contigs were used to obtain one single closed contig.
The assigned chromosomal data contains all of the genes that have orthologues in P. falciparum (in large syntenic blocks [ 30]) plus some P. chabaudi- and rodent-specific genes and expanded gene families primarily located at sub-telomeric ends of assembled chromosomes.
The coverage of the start and the end of the assembled genome maps was usually low, and hence the first and last five signals on the assembled genome maps were ignored.
We added one cDNA to the selection list for each of EST clusters with a minimum depth of 3 ESTs and minimum predicted distance to the 3' end of the assembled EST contig.
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