Sentence examples for end of assay from inspiring English sources

At the end of assay, plate was read against a blank reagent at 570 nm after 10 min shaking.

One hour prior to the end of assay Caspase-Glo 3/7 reagent (Promega, Madison, WI) was added to each well at a 1∶1 ratio according to manufacturers' instructions.

The samples obtained are extremely diluted and most biomarkers are at the low end of assay sensitivity.

At the end of assay (15 min) specimens with spiked PSA show significantly higher fluorescence intensity than those with 0 pM specimen.

Plaques were counted and fitness was expressed as the number of doublings in a 20 hour period according to the equation log2(# of phage at end of assay) - log2(# phage at start of assay).

End-of-assay medium was collected from assays containing cells transfected with different TNC clones, as described above.

The level of MMP-3 release in relation to SNP genotype was measured by enzyme-linked immunosorbent assay of end-of-assay CM.

End-of-assay medium was collected from the assays, protein levels measured and equal concentrations applied to ELISA plates for MMP-1, MMP-2 and MMP-9 (Amersham, Buckinghamshire, UK).

A sandwich enzyme immunoassay kit (R&D Systems Europe Ltd, Abingdon, Oxfordshire) was used to measure total MMP-3 present in end-of-assay CM and was carried out according to the manufacturer's instructions.

At the end of the invasion assay, medium from the four wells of the assay was harvested (referred to as end-of-assay CM), centrifuged to remove cell debris, and stored at -80°C until use.

Since MMP-3 can activate other MMPs involved in mediating tumor invasion, including MMP-9, the relationship between MMP-3 genotype, IPC, and gelatinase levels in end-of-assay CM was analyzed.