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As it turns out, it matters to us, his readers, too: it's often the first question asked at the end of a reading.
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Thus, even preferential mutations at the end of a read will not result in a systematic bias.
These low-quality bases occur preferentially at the ends of reads, with 90% occurring either 50 bp from the 5' end or 300 bp from the 3' end of a read (Figure S1).
While an initial assessment of position within the read indicated that as the SNPs were located towards the end of a read the percentage of monomorphic markers increased, removal of SNPs detected with low quality bases eliminated this trend.
Due to the chemistry of the sequencing reaction, the bases sequenced at the end of a read tend to have higher error rates, resulting in mismatches, indels, or ambiguously identified bases (Ns).
An example of this scenario would be a deletion near the end of a read.
Null sites occurred when neither end of a read aligned to a restriction site.
We also trimmed any mononucleotide run from the end of a read.
This was particularly difficult when the INDEL is located toward either end of a read [ 40].
If the 5' end of a read was not aligned to the genome, the read was eliminated from the analysis.
We considered as covered all the amino acids between the start and the end of a read-ORF alignment.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com