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Apoptotic cells and bodies were visualized by 3′ end labelling.
PCR products were then digested with EcoRI and purified, prior to end labelling with Klenow.
TdT-mediated dUTP nick end labelling assay was performed using DeadEnd™ Fluorometric TUNEL System (G3250; Promega).
The presence of the DNA ladder related well to the end-labelling method in that the least amount of end labelling was seen in samples in which necrotic degradation rather than apoptotic ladders were seen.
Extent of apoptosis was defined morphologically and by the TUNEL (Tdt-mediated dUTP biotin nick end labelling) assay.
We exposed transversal leaf sections to terminal deoxynucleotidyltransferase enzyme-mediated dUTP end labelling (TUNEL).
Similar(23)
TDT-mediated dUTP-biotin nick-end labelling.
Tumour cell apoptosis was assessed by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling.
Terminal deoxynucleotide transferase-mediated dUTP nick-end labelling reaction solution was substituted with TdT-free solution for a negative control.
Increased cell death in the endothelium was shown by elevated labelling of DNA fragments by terminal uridyldeoxynucleotide nick-end labelling.
Nucleosome positioning and DNA accessibility were determined by micrococcal nuclease (MNaseI) and DNaseI digestion, respectively, followed by indirect-end labelling.
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