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Oligonucleotide probes were 32P-labeled using T4 Polynucleotide Kinase 5' End Label System (Promega) and purified using Mini Quick Spin Oligo Columns (Roche).
He left RCA to found Carlton Records in 1957, and though one of his biggest successes came from Squaresville's Anita Bryant ("Paper Roses"), Carlton put points on the board with underrated rocker Jack Scott, R&B singer Big Al Downing, rockabilly Jesse Lee Turner and Godhead girl group the Chantels, whose 1958 stunner "Maybe" was, sadly for Joe, on George Goldner's End label).
CE with mass-spectrometric detection will allow the analysis of sialylated glycans with and without a reducing end label, but not of the nonlabeled neutral glycans.
To determine if apoptotic cells are observed in treated cells, we performed the terminal deoxynucleotidyl transferase-mediated dUTP nick end label (TUNEL) assay using ApopTag kit (Invitrogen) as described previously (Komata et al, 2000).
Stock solutions of DNA containing either a 5′ or 3′ P end label were generated by incubation of a DNA strand with [γP]ATP (PerkinElmer) and T4 polynucleotide kinase (New England Biolabs) or [αP]ATP (PerkinElmer) and terminal transferase (New England Biolabs), respectively.
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Endothelial apoptosis was assessed by TdT-mediated dUTP nick end labeling assay.
transferase dUTP nick end labeling.
Terminal deoxynucleotidyl transferase dUTP nick end labeling.
terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling.
Terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling.
Probes were end labeled with a Biotin 3' End DNA Labeling Kit (Pierce, Rockford, IL).
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