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Consequently, the exposed chromosome end initiates a DDR that results in the activation of a persistent p53-dependent G1 DNA damage checkpoint (Campisi & d'Adda di Fagagna, 2007).
In contrast, when the non-mutated end initiates recombination, then the frequency of incorporating a given mutation into heteroduplex depends on how far the mutation is from the marker.
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While end initiated debonding and peeling mechanisms have been researched extensively, crack initiated debonding has not been studied to the same extent.
Plate end initiated debonding is the far more common failure mode reported, and the research by Smith and Teng (2002) focused on strength models for this failure mode.
The pattern of amplifications observed in the case of the D12 GCR could be explained by a complex mechanism initiating by the original chromosome V chromosome XIV dicentric translocation breaking within the YNL018-019C region and the resulting end initiating a BIR event by priming in the inverted repeated YNL033-034W region on the same arm of chromosome XIV.
Visible cracking started at the loading-end initiated debonding which propagated towards the far end until complete debonding occurred.
In 76.8% of cases, the 3'-ends initiated priming at complimentary sequences of ≥ 2 bp on the new templates.
However, analysis of sequence discrepancies suggests that BLAST was >95% accurate in assigning the correct nucleotide at which the 3'-end initiated priming on the new template.
The uncapped 5′-ends initiated by known miRNAs represented less than 2% of the total unique ends passing the statistical test which suggests that miRNA-independent mechanisms may contribute significantly to the formation of predominant uncapped 5′-ends (Additional file 1: Table S1).
After cell division, S. pombe starts to grow only at old ends for a while before the new ends initiate growth at a point in G2 known as new end take off (NETO) (May and Mitchison, 1995; Mitchison and Nurse, 1985).
The ssDNA at telomeres in cdc13-1 mutants resembles a DSB-end, initiating 5′ 3′ resection to generate a 3′ overhang that is an intermediate of HR, and ssDNA can be detected up to 30 kb from chromosome ends (Booth et al, 2001; Zubko et al, 2004).
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