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He is currently in the process of mapping one end fragment of the huntingtin protein in order to understand how other protein complexes bind to it.
If the communications scenario is such that the type of applications and technologies one of the operators operates on, e.g., shorter-range communications, prefers higher end fragment to upper end fragment, the GSC will probably allocate the higher end fragment to that particular operator, even if the other operator seeks the same end.
The nucleotide sequence of the 2.2-kbp Sal 1 end fragment of the RCN DNA has been determined from a cloned copy of the HindIII O fragment.
The labeled transposon end fragment was purified by electrophoresis in a TBE-buffered 5% polyacrylamide gel, and recovered by the crush and soak method as described [29], [33].
The PCR conditions were: initial denaturation at 98°C for 30 sec, followed by 30 amplification cycles (98°C for 10 sec, 56°C for 20 sec, 72°C for 30 sec), and a final extension step at 72°C for 6 min. A 5' end fragment (16S5'-16S3R) and a 3'end fragment (16S9F-16S3') were cloned in the EcoRV site of the plasmid pBluescript and sequenced using the T7 and T3 universal primers.
The right end fragment was not packaged.
Similar(39)
An inversion occurs when a chromosome breaks in two places and the region between the break rotates 180° before rejoining with the two end fragments.
The nucleotide sequences of both repeated and nonrepeated sequences within this Sal 1 fragment are extremely similar to those of the Sal 1-generated end fragments of the DNas of CPV and vaccinia virus.
We first cloned the partial coding sequences and then obtained the 5' and 3' end fragments.
Fifty Spanish EV13 VP1 3′ end fragments were also submitted under accession numbers AY227284 AY227333.
The 3' end fragments of the digested cDNA was recovered with Dynal beads.
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