To this end, expression constructs were prepared which contained a promoter of a kanamycin resistance gene, the Arabidopsis AAE15 gene, the terminator sequence of a native aas, and the chloramphenicol resistance gene as a selection marker.
To this end, expression plasmids encoding each zipper-containing fiber protein were transfected into HEK 293 cells and fiber expression and trimerization evaluated via western blot analysis.
To this end, expression of some p53 mutants and to a lesser extent wild type p53, lead to increase of average cell area, decrease of dispersion and elongation indices, and re-appearance of actin bundles [8].
To this end, expression of FAK-related nonkinase (FRNK) or small interfering RNA (siRNA) against FAK was used to disrupt the FAK-induced signal transduction pathways in the HNSCC-derived SCC40 and SCC38 cell lines.
To this end, expression ratios were calculated between the reference RNA and the Wilms' tumor data for the SAGE tag counts as well as for Affymetrix HG-U133A GeneChips spot intensities.
To this end, expression of the rate-limiting enzymes in catecholamine (TyrH), serotonin (TrypH) and GABA (GAD) biosynthesis, as well as the vesicular acetylcholine transporter (VAChT), were examined in uninduced (D0) and induced (D6 and D12) MSCs (Fig. 2A).
To this end, expression vectors for the bromodomain testis-specific (Brdt-GFP tag), TPs I and II (HA tag), and protamine 1 (Prm1-red variant of GFP tag) were generated and transfected into the fibroblasts.
Although the effects were often less dramatic than those seen at the 5′ end, expression at the 3′ end can decrease to levels where the main stop codon is not part of the dominant form of the transcript, or not present at all in the data.
More recently, a few high-end expressions have begun to appear.
Rmodel can identify any type of change in mRNA processing, however the bias of 3'-end expression microarrays, combined with the general lack of introns in the 3'-UTR leads to a significant bias towards changes at the 3'-end of the resulting transcripts, which are expected to generate only one segmentation point in the probeset.
