Exact(4)
For bcPCR the gene-specific The same PCR primers (341F and 785R) as for the first PCR reaction were tagged with adapter sequences, specific barcodes and key sequences at the 5′ end, compatible with Ion Torrent sequencing technology (total 60 62 nucleotides per primer).
Hybridization of the primers resulted in a 5′ end that was compatible with NotI and a 3′ end compatible with ApaI.
Both EPEC and SIEC samples presented similar sheath-like structures with a basal body at one end, compatible with the injectisomes reported in EPEC. Figure 4B shows 10 aligned injectisome particles of each preparation to compare their overall structure.
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Similar(56)
Plasmid p416-GAL1 [ 18] was digested with XhoI, blunt-ended with Klenow Polymerase followed by heat-inactivation and digestion with Spe1 creating ends compatible with the digested αMF amplicon.
The basic BER/SSBR pathway involves four key reactions: (1) excision of the base lesion by a DNA glycosylase, (2) end-processing at the SSBs to generate 3′OH and 5′-phosphate ends, compatible with gap-filling synthesis and ligation, (3) gap-filling by a DNA polymerase, and (4) final nick sealing by a DNA ligase33.
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We tested this approach by modifying the sequenced RAD tag protocol [6] in order to create paired-end compatible libraries.
The MfeI restriction enzyme leaves protruding ends compatible with EcoRI terminal ends.
Removal of the weakly bound 4 bp ssDNA stretch creates cohesive ends compatible with those generated by the restriction enzyme KpnI.
With two non-overlapping sets of restriction enzymes that generate cohesive ends compatible with either BglII or AgeI, our new backbone vectors provide greater flexibility and should simplify the future construction of pRS/pRSII plasmids with additional yeast-selectable markers.
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