Sentence examples for end adaptor from inspiring English sources

The enriched RNA was converted into double-strand cDNA by using a cDNA synthesis kit (Invitrogen) following the manufacturer's protocol, except for using the biotin-labeled primer based on the 3' end adaptor sequences for the priming (5' biotin-ATCTAGAGCGGCCGCAATGGCCACTCTGCGTTGATAC).

After removing sequences from remaining ribosomal RNAs, tRNAs, mitochondrial RNAs, sequences without a 3' end adaptor, and sequences shorter than 11 bp, 148,520 sequences were qualified, representing 52,571 distinct 3' ESTs.

End repair was performed, adding A and adaptors in which the cytosines in the paired end adaptor sequence were methylated.

Repaired fragments were ligated with methylated sequencing adaptors using a paired end adaptor oligo kit and oligo mix 5 (Illumina).

End repair was performed, adding A and adaptors, where the cytosines in the paired end adaptor sequence were methylated.

The first comprised 27 cycles (94°C for 30 sec, 66°C for 30 sec, 72°C for 1 min 30 sec) using the end of the adaptors as primers.

In brief, cDNA amplicons (300 750 ngs) underwent end-repair and adaptor ligation processes to generate SMRTbell™ libraries for circular consensus sequencing.

The digested DNA was purified, and ligated with Blunt end adaptors (25 μM, provided with the kit) for overnight at 16°C.

From this step the procedure was similar to the shotgun one, consisting in the nebulization of circular molecules, paired end adaptors ligation and amplification of the library.

Libraries were constructed by end repair, A-tailing, paired-end adaptor ligation, amplification, hybridization, indexing, and enrichment using a SureSelectXT reagent kit, HSQ (Agilent Technologies).

The sheared genomic DNA was examined by Agilent 2100 Bioanalyzer DNA12000 Chip (Agilent Technologies Inc). for size distribution and underwent DNA damage repair/end repair, blunt-end adaptor ligation followed by exonuclease digestion.