Sentence examples for end adapter from inspiring English sources

PCR and blunt end adapter PCR were used unsuccessfully to attempt to close the gaps.

The generated sequence reads were processed to remove the 3' end adapter, if applicable.

After downloading, all sequence reads were first processed to remove the 3' end adapter and the ones with adapter detected and trimmed were called "clean reads".

Following paired end adapter ligation, fragments of approximate size 400-425 bp were gel purified and PCR amplified using the paired end Illumina library PCR primers (primers 1.0 and 2.0).

Rohland and Reich created libraries with as little as 200 ng of DNA/person but report 50% sample loss from their blunt end adapter ligation strategy and therefore recommend using ≥500 ng of DNA/pp [ 16].

The libraries obtained for each sample are then combined and subjected to a second round PCR that adds a barcode and the end adapter sequences required for high-throughput sequencing.

First the 3′ end adapters of reads generated by Illumina Genome Analyzer IIx were trimmed using The Flexible Barcode and Adapter Remover (FLEXBAR, Dodt et al., 2012).

Although blunt-end or single-strand adapters are sealed, the Rnl2 performed better with sticky end adapters (Figure 2E).

Paired end adapters were ligated using Rapid T4 DNA ligase and then size fractionated.

After that, Illumina paired end adapters were ligated to the resulting cDNA fragments.

cDNA fragments were end-repaired and phosphorylated, followed by adenylation of 3′ends and adapter ligation as described [ 34] with the exception of replacing standard paired end adapters with forked adapters containing unique 8 base index sequences.