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Hence, the 2D gel analysis monitors ARS1211 left forks encountering head-on the PDC1-TRX1 transcription units; the right forks instead immediately run off the restriction fragment, preventing the visualization of bubble-like intermediates.
We performed a 2D gel analysis on three additional loci in which forks encounter head-on RNAPII-transcribed units SNR13-TRS311, SOD2-ERG11, and HYP2).
When a replication fork is encountered head on with the transcribed gene, the resulting collision collapses the fork.
Before finding his photographic style, the iconic Love Park in Philadelphia was Angemi's first window into encountering tensions head-on through skateboarding.
These biases may reflect tendencies of adult rats to encounter objects head-on and to actively palpate the whiskers forwards and backwards, but it is difficult to determine the precise patterns of whisker deflections in live animals to use as inputs to the model.
I encountered the robber head-on, and instead of running, I interrogated him.
While crossing the jungle gym, I encountered another child head-on.
Even then you may encounter unexpected resistance or roadblocks, but you'll be better prepared to meet them head-on.
He added: "Alex faced all of the challenges he encountered throughout his life head-on.
Moreover, our findings that another population of Sen1 associates with Nrd1 and binds RNAPII and RNAPIII genomic elements throughout the cell cycle and that Nrd1 does not affect S phase events strongly suggest that Senataxin has evolved at least two functions one in facilitating RNA biogenesis and one in assisting fork progression in head-on encounters with RNAPII-transcribed genes.
This indicates that T. brucei does not avoid head-on encounters between transcription and replication and that such encounters impose a greater impediment to replication than when the two processes are codirectional (Merrikh et al., 2011).
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