Sentence examples for encompassing restrictions from inspiring English sources

The notion of a limitation is broad and elastic, encompassing restrictions on such "basic" actions as lifting one's arm (Nordenfelt, 1997; compare Amundson, 1992: actions "at the level of the person"); on more complex physical activities such as dressing and toileting; and on social activities like working, learning or voting (see Wasserman, 2001).

We tried to produce CAPS markers by designing primers encompassing restriction enzyme recognition sites using Primer 3 if restriction enzyme recognition sites were present.

Explanatory variables encompass restrictions to specified centres or specialists, previous approval by a medical advisor of the health insurer or the requirement for a registry.

The sequence encompassing different restriction sites on pGl3-Basic vector are given in Additional file 4: Figure S7.

Optimizing the TJ tightness of hCMEC/D3 cell monolayers still remains a major challenge in order to provide an in vitro model that might recapitulate all the characteristics of human BBB, encompassing permeability restriction with appropriate molecular exclusion and functional efflux and influx transport systems.

A randomly 32P-labeled (Rediprime II Random Prime Labelling System, Amersham Biosciences) restriction fragment encompassing all of exons 1 and 2, all of the ORF and a part of exon 3 (XbaI-fragment) was used as a PrP probe.

Upon initialization and depending on the depth of the HiC dataset, the software splits the contigs into smaller pieces/bins encompassing at least 2 restriction fragments.

Plan your meals so they encompass any food restrictions and recommended daily intake amounts.

To genotype the G894T polymorphism in the exon 7, we performed a polymerase chain reaction (PCR) amplification of 206 bp fragment encompassing the variant, followed by restriction fragment length polymorphism (RFLP) using the restriction endonuclease Mbo1 (Promega).

For the restriction analysis, cDNA encompassing the respective mutation site was amplified using mutation-specific primers (Online Resource).

Two gene-specific primers, Lc4ORFs and Lc4ORFas (Table 1) encompassing the BamH1 and EcoR1 restriction sites were designed to amplify Lca-KPVQ ORF using the Advantage®2 PCR enzyme system (Clontech), which was then ligated into the TOPO® TA PCR 2.1 vector (Invitrogen) and the construct was transformed into TOPO 10 cells.