Briefly, capture oligos were designed to encompass putative SNVs, SVs, and indels and used as input for NimbleGen Seqcap EZ solution bait sets.
A synthetic chaperone consisting of the granzyme B ER signal peptide and a domain encompassing putative N-linked glycosylation sites in granzyme B is insufficient for payload transfer to target cells, whereas full-length granzyme B is sufficient for payload delivery.
Therefore, a precise characterization of the microsynteny blocks that encompass putative GRDs in the genus Drosophila is still lacking.
First, we extended the regions which encompass putative duplications and which were not allowed to feature markers.
The amplicons used in the present study were designed in order to cover a broad spectrum of regions within DEFB encompassing putative promoters, exons and introns and intergenic parts.
In order to investigate the exon/intron structure of the cotton GaSus genes, seven genomic DNA sequences, encompassing putative overall coding regions of GaSus1 to 7, were cloned from the G. arboreum genome.
To our surprise, stage 3 (Flowering) and stage 6 (Fruit drop) both possessed obvious clusters that encompassed putative representatives for almost all the enzymes in the biosynthesis pathway, suggesting that these two developmental stages are important points of ginsenoside biosynthesis in the developing plant.
The AKAP sequence of BIG1 is critical for this interaction, but the association between BIG1 and mDpy-30 is likely further strengthened by the presence of an integral DCB/HUS dimerization region of BIG1 that encompasses the putative AKAP sequence.
The H2 helix is less conserved, but typically encompasses a putative ribosome-binding site in the 3′ portion.
Accordingly, the PxxDY motif encompasses a putative internalization motif (YxxI/L), albeit this sequence has been described to behave only as a weak internalization signal in the first report of CD3ε endocytosis [ 58].
