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To produce a construct enclosing all elements of the regulatory region a (-1895/+106) fragment, containing proximal enhancer and promoter region of vole Oct4 gene, was cloned into plasmid pDE2 at HindIII site (pDEH6 construct).
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By enclosing all such data in a single parent element with a uniquely defined dictionary reference a low-memory streaming parser can extract the data without the need for complex document manipulation.
This is the overwhelming issue that encloses all others.
Another set of boundary condition is the mass flux boundary condition, which defines the fluxes in mass at the nodes enclosing faces of elements.
Each cell in this model is an individual unit containing several sub-cellular elements, such as the elastic plasma membrane, enclosed viscoelastic elements that play the role of cytoskeleton, and the viscoelastic elements of the cell nucleus.
Hereafter, parentheses in construct designations enclose the elements flanked by the frt or lox sites.
The translation of Control elements is more complicated, because they are translated into a transition or a reaction depending on enclosed Control elements.
Each cell in this model is an individual unit containing several subcellular elements, such as the plasma membrane, enclosed by viscoelastic elements that play the role of cytoskeleton, and the viscoelastic elements of the cell nucleus.
For each element along the path, we perform the above ray-tracing test and calculate the distance (d) between the current photon position (p) to the exit point (px) of the enclosing element along the propagation vector (c).
As the photon moves along its trajectory, the photon packet weight is attenuated exponentially based on the Beer-Lambert law [ 1] using the absorption coefficient (μ a) of each enclosing element.
For nearly all Control elements, a ControlType is assigned describing the relationship between the enclosed elements (i.e. activating, inhibiting).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com