Participating laboratories were instructed to follow the enclosed testing instructions; subculture the test strains, and propagates the quality control strains prior to performing the susceptibility method that was routinely used by their laboratory.
Briefly, individual animals were placed in small enclosed testing arenas (20 cm × 18.5 cm × 13 cm, length × width × height) on top of a wire mesh floor and allowed to acclimate for a period of at least 90 min. The DPA device was then positioned so that the filament was directly under the plantar surface of the ipsilateral hind paw of the animal and tested three times per session.
For example, in the 9-wasp treatment, after 2 hours, ostiole closure was tested by using a translucent organdy bag to enclose a test syconium with a newly-caught wasp.
Quality control is provided by testing three control sera, enclosed in every test kit.
An insulated chamber was used to enclose the test samples during the treatment for maintaining isobaric and quasi-isothermal processing conditions.
Mice were placed in the enclosed quadrant and tested for 5 min. Test sessions were recorded and scored later for time in open area, head dip frequency and number of open area entries.
Unlike conventional nucleic acid amplification tests (NAATs), Xpert is unique because sample processing and PCR amplification and detection are integrated into a single self-enclosed test unit, the GeneXpert cartridge.
A ball is permanently enclosed if, when tested in accordance with 16 CFR 1500.53, it is not removed from the outer container.
A marble is permanently enclosed if, when tested in accordance with 16 CFR 1500.53, it is not removed from the toy or game.
Therefore, analysis of this moderate tsunami contributes toward tsunami assessment and awareness in this enclosed basin and tests the functional performance of TSPs in the NEAMTWS region.
