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The total RNA was purified and treated with DNase treated using NucleoSpin RNA L (Macherey-Nagel GmbH & Co KG, 52355, Düren, Germany) following the enclosed protocol.
The final extract was further purified using the RNeasy Plant Mini Kit following the enclosed protocol from the step where the extract is loaded on a QIAshredder column.
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The total RNA was extracted following the protocol enclosed in RNA prep pure kit (Tiangen Biotech, Beijing, China).
The DCD-III protocol enclosed limitation of life sustaining therapies (LLST) in both the intensive care unit (UCI) and the operating room (OR), and included two strategies (Strategy A: rapid surgery and Strategy B: cannulation, Figure 2).
All subjects signed the written Informed Consent Form enclosed in the protocol approved by the Hospital Ethics Committee from the State University of Rio de Janeiro, in accordance with the Helsinki Declaration.
Total RNA was extracted using TrIzol® (Invitrogen) according to the reagent-enclosed protocol.
Figure 2: Minimal sample-processing protocol performed in an enclosed volume is amenable to automation and scaling.
Figure 2 : Minimal sample-processing protocol performed in an enclosed volume is amenable to automation and scaling.
All neat DBC powder was handled by a trained carcinogen specialist in an enclosed glovebox, per the OSU Extreme Carcinogen handling protocol.
Male Wistar rats were trained in the PMDAT (plus-maze with two open and two enclosed arms) using a standard or a modified protocol.
Twelve patients were treated with adjuvant chemotherapy after radiation with nine of them being enclosed within or treated in analogy to multimodal protocols conducted by the German Society for Pediatric Hematology and Oncology (GPOH, HIT-Studies).
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