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This study showed that the BSA/GA emulsification-coacervation is an efficient and promising technique for CN encapsulation according to encapsulation efficiency and facility of the method.
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The encapsulation efficiencies were compared and are shown in figure 2. We observed an increase of encapsulation efficiency according to the number of freeze-thaw cycles without significant denaturation of the enzyme.
It was determined that the initial amount of α-tocopherol showed a cubic effect on the maximum encapsulation efficiency according to the microfluidisation cycles used.
Fig. 5 Encapsulation efficiency of DOX in dual drug-encapsulated nanoliposomes according to various drug-loading conditions: drug-loading conditions for each experimental group (a) and drug encapsulation efficiencies of the experimental groups (b) (n = 3, the data are presented as mean ± SEM).
However, message translation is challenged by the encapsulation of data according to different middleware protocols, e.g., SOAP message encapsulated within HTTP for Web Services.
Fig. 4 Encapsulation efficiency of DOX in dual drug-encapsulated nanoliposomes according to DOX-loading methods and ammonium sulfate (AS) concentrations: CA citric acid (n = 3, the data are presented as mean ± SEM).
The concentration of DOX in the liposomes was measured using a UV-vis spectrophotometer at 497 nm (UV-mini; Shimadzu, Tokyo, Japan), and the encapsulation efficiency was calculated according to the following equation: Encapsulation efficiency % = F t F i × 100 (100
In this study, load-carrying performance and hydrostatic tests of the developed encapsulation anchor were undertaken according to the Korean and U.S. testing standards.
In vitro encapsulation assay was performed according to previous report [33].
The encapsulation assay was done according to Sorrentino et al. [42].
Encapsulation efficiency was determined according to the adapted method previously described [ 24].
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