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The radiolabeling efficiency increases with the polymer up to a value of 1.1 mg/mL, after which the encapsulated percentage remains constant, reaching a plateau at about 30%% (Fig. 3a).
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This percentage encapsulated was determined after lysis of the prepared liposomes with ethanol/chloroform (1 : 1) and sonication for 10 minutes.
Encapsulation efficiency was determined as the percentage verbascoside encapsulated in liposome to the original amount of verbascoside added.
The effect of lecithin cholesterol molar ratio on the percentage drug encapsulated was investigated.
Once the particle size and morphology was characterized, the mass percentage of BSA encapsulated as well as the encapsulation efficiency of the model drug was determined (Table 2).
Encapsulation efficiency was defined as the percentage of pDNA encapsulated in NPs with respect to the initially added amount of pDNA.
Table 1 Characterization of the liposomes by ICP-OES Sample P (mM) As (mM) Ni (mM) As/P Lipo-ATO 2.37 ± 0.03 0.78 ± 0.23 2.71 ± 1.63 0.33 Control lipo 2.22 ± 0.04 ND 2.81 ± 0.12 Data are shown as means ± standard deviations of three replicate measurements ND lower than detection limit Fig. 1 Percentage of ATO encapsulated in the liposome over a 1-month period of storage at 4 °C.
Capecitabine was successfully encapsulated into semi-IPN microspheres and percentage of encapsulation efficiency varied from 79to87879to87
According to protocol, the amount of BSA released was expressed as a percentage of total BSA encapsulated in chitosan microparticles as calculated from the LE value.
A membrane integrity assay (LIVE/DEAD® Viability/Cytotoxicity Kit) of cells encapsulated in gels (Fig. 6a) showed a higher percentage of living cells in gels polymerized for 1 minute (87 ± 2%) in comparison to 5 minutes (81 ± 4%) at day 3 in culture.
Only at higher amounts a decrease is seen which is explained by the fact that if there is an excess of free tropolone, i.e., percentage-wise less 111In-labeled tropolone will be encapsulated, assuming that both species behave in a similar way (i.e., have a similar D value).
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