In vitro transcription assays and measurements of promoter activity using the overexpression of a single sigma factor gene and the transcriptional fusion of the promoter to the gfpuv reporter gene enabled us to reliably define the sigma factor dependency of promoters.
The MeDIP procedure combined with CGI microarray technology enabled us to reliably and efficiently profile the methylation states of a wide range of CpG islands in multiple samples.
Use of different computational approaches enables us to reliably recognize distant relationships, especially when they converge to a common assignment because the methods are often complementary.
These important design features enabled us to reliably train and test a classifier and to dissociate the multi-voxel patterns of both schema components (rule-based associations, low-level visual features).
A validated statistical model enabled us to reliably predict that from 2010 to 2025 the number of new cases would reach 46,600, with an evident downward shift in the age of onset.
The utilization of the dual-luciferase imaging approach enabled us to reliably quantify the magnitude of down-regulation of histone methylation in tumor tissue induced by treatment of mice with histone methyltransferase inhibitors in vivo.
By training and testing subjects on schema material across consecutive days, we achieved near-ceiling performance that enabled us to reliably train and test a classifier and to dissociate the multi-voxel patterns of both schema components (rule-based associations, low-level visual features).
Although coding sequences were not used as sources of STS markers in this study, the stringent requirements used to select STS for RH mapping enabled us to reliably identify homologous loci within HSA6 for all but two of the bovine STS markers mapped in this study.
This simple antenna array enabled us to reliably reach directional accuracies of <3°, determined by tracking a person carrying a radio transmitter at the distance bats were foraging, in a blind experimental setup.
Mutations are considered non-neutral if they cause a >1.3-fold change in phosphotriesterase activity in lysate compared to the parent background because, in our screening system, this cut-off enabled us to reliably identify improved variants.
